منابع مشابه
Increased Efficiency of GroE-assisted Protein Folding
This study addresses the role of ATP-bound and free Mg and Mn ions in the activation and modulation of chaperonin-assisted refolding of urea-denatured malate dehydrogenase. As compared with Mg, Mn ions caused a significant increase in the rate of GroE-assisted malate dehydrogenase refolding and, concomitantly, a decrease in the rate of ATP hydrolysis. Moreover, Mn increases the affinity of GroE...
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In the processes of protein synthesis and folding, newly synthesized polypeptides are tightly connected to the macromolecules, such as ribosomes, lipid bilayers, or cotranslationally folded domains in multidomain proteins, representing a hallmark of de novo protein folding environments in vivo. Such linkage effects on the aggregation of endogenous polypeptides have been largely neglected, altho...
متن کاملDual Function of Protein Confinement in Chaperonin-Assisted Protein Folding
The GroEL/GroES chaperonin system mediates the folding of a range of newly synthesized polypeptides in the bacterial cytosol. Using a rapid biotin-streptavidin-based inhibition of chaperonin function, we show that the cage formed by GroEL and its cofactor GroES can have a dual role in promoting folding. First, enclosure of nonnative protein in the GroEL:GroES complex is essential for folding to...
متن کاملScanning and escape during protein-disulfide isomerase-assisted protein folding.
During oxidative protein folding, efficient catalysis of disulfide rearrangements by protein-disulfide isomerase is found to involve an escape mechanism that prevents the enzyme from becoming trapped in covalent complexes with substrates that fail to rearrange in a timely fashion. Protein-disulfide isomerase mutants with only a single active-site cysteine catalyze slow disulfide rearrangements ...
متن کاملImportance of Protein Folding Sequence-Structure-Function
Protein Structure Prediction (II): Fold Prediction “Understanding protein structure, function and dynamics ranks among the most challenging and fascinating problems faced by science today. Since the function of a protein is related to its three dimensional structure, manipulation of the latter by means of mutation in the protein sequence generates functional diversity. The keys that will help u...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1995
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.1995.tb02423.x